Protein Purification
Promega High Capacity Magne Streptavidin Beads, 3ml
High Capacity Magne Streptavidin Beads are magnetic affinity beads with high specificity and high capacity for binding biotinylated antibodies and proteins. Goat Anti-Human Biotinylated IgG is 0.5mg/ml in PBS (pH 7.4) with 0.1% sodium azide.
Promega HaloTag™ Mammalian Pull-Down Systems
The HaloTag™ Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
Promega HaloTag™ Biotin Ligand
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
Promega NanoBRET™ Nano-Glo™ Detection System
The NanoBRET Nano-Glo™ Detection System provides the NanoBRET Nano-Glo™ Substrate used by NanoLuc Luciferase to generate the donor signal and the HaloTag™ NanoBRET 618 Ligand for the fluorescent energy acceptor.
Promega HaloTag™ Complete Pull-Down System
The HaloTag™ Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
Promega NanoBRET™ PPI Flexi™ Starter System
Protein:Protein Interaction (PPI) Assays use NanoLuc Luciferase and HaloTag™ protein fused to target proteins of interest to enable sensitive, reproducible detection of protein interactions in the natural cellular environment.
Promega Protease Inhibitor Cocktail
Protease Inhibitor Cocktail is used to prevent protein degradation after lysing cells. The product is a mixture of six different protease inhibitors with different target protease specificities.
Promega pFN29K His6HaloTag™ T7 Flexi Vector for E. coli and Cell-Free Protein Expression
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
Promega MagneHis™ Protein Purification System
The MagneHis Protein Purification System provides a magnetic-based, rapid method for the purification of polyhistidine- or HQ-tagged, expressed proteins.
Promega HaloTag™ PEG-Biotin Ligand
The HaloTag™ Technology enables covalent protein labeling and immobilization in vivo and in vitro.
Promega NanoBRET™ Nano-Glo™ Substrate
The NanoBRET™ Nano-Glo(R) Substrate is the substrate used by NanoLuc(R) Luciferase to generate the donor signal in several NanoBRET™ Assays.
Promega HaloTEV™ Protease
HaloTEV Protease (81kDa) is a fusion between the HaloTag™ protein and TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV site, a specific seven-amino-acid sequence (ENLYFQ(G/S)).
Promega Proteasome-GLO™ Cell-Based Assays
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
Promega E. coli S30 T7 High-Yield Protein Expression System
The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.